Browsing Faculty of Science e-Theses Collection by Author "Chidzwondo, Farisai"

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Expression of the cDNA Encoding the Pterocarpus angolensis (Mukwa Tree)-Seed Lectin in Escherichia coli and Site-Directed Mutagenesis of the Sugar-Binding Specificity Loop

Chidzwondo, Farisai (2014-06-16)

The mannose/glucose specific lectin from Pterocarpus angolensis (mukwa tree) seeds was expressed in Escherichia coli using the pBAD expression system. The expression vector pBADMycHisA was digested with NcoI and filled-in ...