Model for Assessment of Endothelial Cell Function and Viability Using the MTT Dye Test and {3H}

Abstract

A highly specialised cell layer made of vascular endothelial cell (EC) lines all blood vessels and lymphatics.1 The function of this cell layer appears to vary according to anatomical site and size of the vessel.2 However, the basic function of supporting either anticoagulant orprocoagulant activities, depending on the local conditions and circumstances, are essentially similar. Any disruption of the equilibrium that exists between the endothelial cell and the circulating blood may result in clinically significant thrombosis or haemorrhage.3'4 Such pathophysiological insults include bacterial lipopolysaccharide (LPS) which may induce endotoxic septicaemia associated with disseminated intravascular coagulation,5 as well as the infusion of a variety of drugs into the vascular component.6 To investigate the effect of such agents and toxins on the endothelial cells, it is necessary to monitor metabolic and functional aspects of the cells’ behavior. This may provide an in vitro model for studying clinical conditions.