University of Zimbabwe Institutional Repository

Model for Assessment of Endothelial Cell Function and Viability Using the MTT Dye Test and {3H}

Show simple item record

dc.creator Mbanya, D.N.
dc.creator Kesteven, P.J.
dc.creator Saunders, P.W.
dc.date.accessioned 2015-06-12T09:53:53Z
dc.date.accessioned 2015-12-08T10:55:02Z
dc.date.available 2015-06-12T09:53:53Z
dc.date.available 2015-12-08T10:55:02Z
dc.date.created 2015-06-12T09:53:53Z
dc.date.issued 2001-07
dc.identifier Mbanya, D.N. , Kesteven, P.J. , Saunders, P.W. (2001) Model for Assessment of Endothelial Cell Function and Viability Using the MTT Dye Test and [3H], CAJM vol. 47, no.7. (pp. 177-81) UZ, Avondale, Harare: Faculty of Medicine.
dc.identifier 0008-9176
dc.identifier http://opendocs.ids.ac.uk/opendocs/handle/123456789/6322
dc.identifier.uri http://hdl.handle.net/10646/2094
dc.description.abstract A highly specialised cell layer made of vascular endothelial cell (EC) lines all blood vessels and lymphatics.1 The function of this cell layer appears to vary according to anatomical site and size of the vessel.2 However, the basic function of supporting either anticoagulant orprocoagulant activities, depending on the local conditions and circumstances, are essentially similar. Any disruption of the equilibrium that exists between the endothelial cell and the circulating blood may result in clinically significant thrombosis or haemorrhage.3'4 Such pathophysiological insults include bacterial lipopolysaccharide (LPS) which may induce endotoxic septicaemia associated with disseminated intravascular coagulation,5 as well as the infusion of a variety of drugs into the vascular component.6 To investigate the effect of such agents and toxins on the endothelial cells, it is necessary to monitor metabolic and functional aspects of the cells’ behavior. This may provide an in vitro model for studying clinical conditions.
dc.language en
dc.publisher Faculty of Medicine, University of Zimbabwe (UZ.)
dc.rights http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.rights University of Zimbabwe (UZ).
dc.subject Science and Society
dc.title Model for Assessment of Endothelial Cell Function and Viability Using the MTT Dye Test and {3H}
dc.type Article


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search UZeScholar


Advanced Search

Browse

My Account

xmlui.statisticsGoogleAnalytics.Navigation.title