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Expression of the cDNA Encoding the Pterocarpus angolensis (Mukwa Tree)-Seed Lectin in Escherichia coli and Site-Directed Mutagenesis of the Sugar-Binding Specificity Loop
(2014-06-16)
The mannose/glucose specific lectin from Pterocarpus angolensis (mukwa tree) seeds
was expressed in Escherichia coli using the pBAD expression system. The expression vector
pBADMycHisA was digested with NcoI and filled-in ...